Cambridge Healthtech Instituteの第2回年次
Maximizing Protein Production Workflows
(タンパク質生産最大化のワークフロー)
徹底的なラボ管理End-to-End Laboratory Management
2023年5月18〜19日 EDT(東部夏時間)
5月16日(火)
Recommended Dinner Short Course6:30 pm
SC7: Use and Troubleshooting of Eukaryotic Expression Systems
*Separate registration required. See short courses page for details.
5月18日(木)
Registration and Morning Coffee7:30 am
WORKFLOW MANAGEMENT: MEETING YOUR CUSTOMERS' NEEDS BY INCREASING PRODUCTION EFFICIENCY
ワークフローマネジメント:生産効率を高め、顧客のニーズに応える
Protein Production Lab Challenges: Methodologies, Strategies, and the Art of Managing Multiple Projects
Richard Altman, MS, Field Application Scientist, Life Science Solutions, Thermo Fisher Scientific
Protein production laboratories provide crucial support to drug discovery efforts. As we would expect, there are numerous challenges in the effective operation of these critically needed facilities. This panel discussion will focus on the concepts, technologies, and strategies necessary to meet the ever-increasing need for recombinant proteins.
- How to build an effective expression facility
- Prioritizing projects or asking the right questions
- Total workflow efficiency
- Engaging and developing team members
- The importance of tech development to long-term success
David Blum, PhD, Director, Bioexpression & Fermentation Facility, Biochemistry & Molecular Biology, University of Georgia

Alexandra Martine, Project Leader, Cell Culture Automation, Selexis
In an effort to improve cell line development processes traditionally performed in Erlenmeyer flask and spin-tube, Selexis has imagined and developed customized and flexible solutions for a fully automated cell culture incubation and passaging process. At the limit between lab automation and industrial robotization, implementing such innovative and customized platform provides high quality processes, precision and process consistency while improving operational efficiency.
Coffee Break in the Exhibit Hall with Poster Viewing10:00 am
WORKFLOW MANAGEMENT: ENHANCING QUALITY CONTROL PROCESSES
ワークフローマネジメント:品質管理工程の強化
Standardizing Methodologies for High-Quality Recombinant Protein Production
Dominic Esposito, PhD, Director, Protein Sciences, Frederick National Laboratory
Generating high-quality, reproducible recombinant proteins is a significant challenge facing the protein production field. The FNL STAR TREC initiative aims to assist in standardization of protein production SOPs and quality control, with the goal of helping to improve reproducibility and minimize financial costs and time wasted in support of basic research and early-stage drug discovery efforts. We will explore ways in which this initiative can guide researchers to improve protein quality and ensure consistency across laboratories and experiments.
Industrial Quality and Academic Creativity: How to Get the Best of Both Worlds
Bjorn Voldborg, MSc, Head, National Biologics Facility, DTU Bioengineering, Technical University of Denmark
The National Biologics Facility (NBF) at the Technical University of Denmark is based on a decade of cutting edge research into the generation of the next generation of CHO cells for industrial production of therapeutics. Throughout the program a strict focus has been on adhering to high quality and documentation levels, while maintaining the flexibility and creativity from the academic setting. The NBF is working as a CRO, keeping the quality levels and documentation requirements required to perform R&D projects for biotech, start-ups and pharma, and functions as consultant for early start-ups to ensure regulatory acceptable cell lines and data.
Luncheon in the Exhibit Hall and Last Chance for Poster Viewing12:10 pm
OPTIMIZING WORKFLOWS WITH AUTOMATION
自動化によるワークフローの最適化
Optimization of Our Rapid Antibody and Protein Therapeutic Omni Robot (RAPTOR) to Improve Throughput
Ayla O. Sessions, PhD, Associate Principal Scientist, Biologics Discovery & Engineering, Merck Research Labs
While advances in lab automation have improved transfection efficiencies and throughput, the purification step is a bottleneck while the cumulative costs of producing thousands of samples can be prohibitive. We have optimized our RAPTOR platform to use cheap, non-proprietary reagents in our methods and leveraged a custom magnetic workflow that captures magnetic affinity beads directly from cell cultures. Optimized elution methods allow for higher protein recovery from these affinity purifications.
Semi-Automated Multi-Host Mid-Scale Expression and Purification Platform
Inna Zilberleyb, Scientist 4, Biomolecular Resources, Genentech, Inc.
We have built a multi-host mid-scale recombinant protein expression platform to accelerate drug discovery research at Genentech. This platform enables quick triage of challenging proteins and complexes for biochemical and structural screening. Our semi-automated workflow leverages in tip affinity chromatography technology, integrated with robotic liquid handlers, and SEC, to purify multiple samples in parallel. The mid-scale platform provides small but sufficient quantities of proteins for biochemical characterization, assay development, and negative stain EM screening.
Establishing a Workflow for Modern Mammalian Expression Platforms
Iman Farasat, PhD, Director, Biologics Discovery, Janssen R&D LLC
Transfection and purification are typically the first identified targets as rate-limiting steps for building mammalian-based HT protein expression workflow. However, with increase in number of samples or expression scale, other factors become visible as rate-limiting steps such as waste management, safety, inputs/outputs, task scheduling, and data handling. Here, we introduce our end-to-end data and hardware automation platform for expressing 100s of proteins at medium scale (~40ml cell culture) in three cell lines (ExpiCHO-S, Expi293, and CHO-K1).
Networking Refreshment Break2:50 pm
THINK TANKS - IN-PERSON ONLY
シンクタンク - 対面参加のみ
- What challenges have we faced?
- What types of improvements in expression hosts we have discussed here today?
- What might address the future and what is needed?
Join a group (topic of your choice) to share and experience and hear what others have learned:
1) Issues and challenges with current expression systems
2) New expression systems and process improvements
3) Parallel simultaneous testing of expression systems
4) Issues and challenges with end-to-end protein production
During the Think Tank Table discussions, we shared our experiences and working solutions for end-to-end protein production workflows. Now as a collective community, let’s hear from the table facilitators as they share key discussion points and strategies, and provide a wrap-up of their table’s discussion. What can we take away and apply?
Close of Day4:20 pm
5月19日(金)
Registration Open7:00 am
INTERACTIVE DISCUSSIONS
インタラクティブディスカッション
Interactive Discussions are informal, moderated discussions, allowing participants to exchange ideas and experiences and develop future collaborations around a focused topic. Each discussion will be led by a facilitator who keeps the discussion on track and the group engaged. To get the most out of this format, please come prepared to share examples from your work, be a part of a collective, problem-solving session, and participate in active idea sharing. Please visit the Interactive Discussions page on the conference website for a complete listing of topics and descriptions.
Combining the Benefits of Academia and Industry: Get the Best of Both Worlds
MEETING PRODUCTION CHALLENGES: DOING MORE WITH LESS
生産上の課題への対応:より少ないリソースでより多く生産する
Unique Challenges and Robust Solutions for Protein Production at an mRNA Company
Ethan Dunn, Manager, Protein Sciences, Moderna, Inc.
The Protein Sciences group at Moderna is responsible for delivering recombinant proteins to support the over 40 mRNA development programs and research projects, from infectious and rare diseases to oncology and autoimmunity, from early platform research to mRNA manufacturing. Close collaboration, attention to detail, and a productive protein production team utilizing a robust toolbox is required to deliver. Some of our challenges, learnings, and future directions will be presented here.
The Daft Punk Approach to Maximizing Protein Production - Faster, Better, Stronger via Leveraging Open Source Robotics, Optimal Scaling, and High Throughput Analytics
Lauren P. Carter, Principal Research Scientist & Engineer, Biochemistry, University of Washington
The Institute for Protein Design has developed powerful processes for computational protein design, most recently the Diffusion model, which combines structural prediction networks with generative diffusion with the ability to generate highly accurate designs optimized for soluble expression. This results in a high numbers of proteins requiring experimental validation. The IPD has developed methods to express, purify, and characterize these designed proteins that can keep pace with design velocity. These methods include utilization of open source robotics, intentional scaling of culture volumes and fractionation for streamlined operations, and high throughput analytical methods for the evaluation of protein aggregation and oligomerization.
Managing People, Priorities, and Proteins: Challenges and Solutions in Protein Science when Facing Headwinds
Jessica Williamson, PhD, US Protein Sciences Lead, UCB
Protein Sciences at UCB makes non-antibody proteins to support drug discovery research across our global organization. Like many, we have navigated retention challenges with the Great Resignation and work/life balance during the global pandemic. Managing a protein science team not only means balancing resources and producing the highest quality proteins, it also means creating growth opportunities for scientists and innovating new methods and technologies to do more with less.
Building Your Own Bioreactor to Increase Throughput for Process Development
David Blum, PhD, Director, Bioexpression & Fermentation Facility, Biochemistry & Molecular Biology, University of Georgia
Bioreactors for process development typically cost between $30,000 to $100,000 making acquisition nearly impossible for startups with low cash flow. We are developing a do-it-yourself (DIY) Bioreactor to address this problem. The system utilizes off-the-shelf software from BlueSens and can incorporate a range of parts that are easily accessible reducing the cost by 5 to 10 fold.
Sponsored Presentation (Opportunity Available)10:00 am
Networking Coffee Break10:30 am
THINK TANKS - IN-PERSON ONLY
シンクタンク - 対面参加のみ
- What challenges have we faced?
- What types of improvements we have discussed here today?
- What might address the future and what is needed?
Join a group to share and experience and hear what others have learned:
1) Workflow vs technology development?
2) Scale-up: when and how to go from research to manufacturing?
3) Doing more with less: how do you test new methods and workflows without blowing up your annual budget?
4) Keeping staff motivated and engaged?
5) Tearing down silos: how do you foster cross-functional collaborations to innovate and improve?
During the Think Tank Table discussions, we shared our experiences and working solutions for end-to-end protein production workflows. Now as a collective community, let’s hear from the table facilitators as they share key discussion points and strategies and provide a wrap-up of their table’s discussion. What can we take away and apply?
Close of Maximizing Protein Production Workflows Conference12:30 pm
* 不測の事態により、事前の予告なしにプログラムが変更される場合があります。
2023年 プログラム
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