Co-Located Conference AgendasFlow Chemistry Asia 2023 | Lab-on-a-Chip and Microfluidics Asia 2023 |
2023年10月5日(木)08:00 | Conference Registration, Materials Pick-Up, Coffee and Tea in the Exhibit Hall カンファレンス受付、資料受け取り | | Session Title: Conference Opening Session セッションタイトル:カンファレンスオープニングセッション |
| | | Session Chairperson: Dr. Claudia Gartner, CEO microfluidic ChipShop GmbH Germany セッション議長:Claudia Gartner博士、ドイツmicrofluidic ChipShop社CEO |
| | 09:00 |  | カンファレンス議長 Welcome and Introduction by Conference Chairperson: Emerging Themes in Microfluidics 2023 カンファレンス議長による挨拶:マイクロフルイディクスにおける新たなテーマ2023年 Claudia Gartner, CEO, microfluidic ChipShop GmbH, Germany
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| 09:30 |  | 基調プレゼンテーション Nanobiodevices, Quantum Life Science, and AI for Future Healthcare ナノバイオデバイス、量子生命科学、および未来のヘルスケアに向けたAI Yoshinobu Baba, Professor, Nagoya University, Japan
We have investigated nanobiodevices, quantum life science, and AI for biomedical applications and healthcare. Nanowire devices are extremely useful to isolate extracellular vesicles from body fluids and vesicle-encapsulated microRNA analysis. The device composed of a microfluidic substrate with anchored nanowires gives us highly efficient collections of extracellular vesicles in body fluids and in situ extraction for huge numbers of miRNAs (2,500 types) more than the conventional ultracentrifugation method. Nanowire devices gave us the miRNA date for several hundred patients and machine learning system based on these miRNA data enabled us to develop the early-stage diagnosis for lung cancer, brain tumor, pancreas cancer, liver cancer, bladder cancer, prostate cancer, diabetes, heart diseases, and Parkinson disease. Nanowire-nanopore devices combined with AI (machine learning technique) enable us to develop mobile sensors for SARS-CoV-2, PM2.5, bacteria, and virus in the environment. MEXT Quantum Leap Flagship Program (Q-LEAP), which I lead, has been developing biological nano quantum sensors, quantum technology-based MRI/NMR, and quantum biology and biotechnology. Nanodiamonds, with nitrogen-vacancy centers, and quantum dots are applied to develop quantum sensors for quantum switching intra vital imaging for iPS cell (induced pluripotent stem cells) based regenerative medicine, and quantum photo immuno-therapeutic devices for cancer. Multifunctional quantum nanoparticles were developed for fluorescence/MR bimodal in vivo imaging-guided potothermal-intensified chemodynamic synergetic therapy of targeted tumors. |
| 10:15 |  | 基調プレゼンテーション Microfluidic Wearable Sensors for Healthcare and Health Metaverse Applications ヘルスケアとヘルスメタバースアプリケーションのためのマイクロ流体ウェアラブルセンサー Chwee Teck Lim, NUS Society Chair Professor, Department of Biomedical Engineering, Institute for Health Innovation & Technology (iHealthtech), Mechanobiology Institute, National University of Singapore, Singapore
The future of healthcare wearables lies in continuous sensing and monitoring in an unobtrusive manner. However, for use as medical wearables, it is important to confer flexibility and stretchability, while maintaining its sensitivity and robustness. Here, we develop novel liquid metal-based microfluidic and microtubular sensors that possess high flexibility, durability, and sensitivity. The sensors comprise a soft elastomer-based microfluidic template encapsulating a conductive liquid metal which serves as the active sensing element of the device. This sensor is capable of distinguishing and quantifying the various vital signals as well as bodily movements it is subjected to. We demonstrated healthcare applications of our sensors in rehabilitation, artificial sensing, disease tracking such as that for diabetic patients and virtual reality training as well as rehabilitation in the health metaverse. Overall, our work highlights the potential of the liquid-based microfluidic sensing platforms in a wide range of healthcare applications and further facilitates the exploration and realization of functional liquid-state device technology. |
| 10:45 | Mid-Morning Coffee and Tea Break and Networking in the Exhibit Hall 休憩および展示ホールでのネットワーキング | 11:15 |  | 基調プレゼンテーション Chemically Programmable Isothermal PCR Enables Rapid Nucleic Acid-based Bioanalysis at the Point of Need 化学的にプログラム可能な等温PCRにより必要に応じて行える迅速な核酸ベースバイオ分析 Victor Ugaz, Professor & Interim Department Head, Texas A&M University, United States of America
The polymerase chain reaction (PCR) and its variants are analytical gold standards for nucleic acid-based testing that play a critical role in diagnosing and monitoring infectious diseases. But robust portable PCR-based testing at the point of need (PON) remains elusive, especially in resource-limited settings, because the required thermocycling instrumentation is neither amenable to nor validated for operation in a portable format. This presentation describes a microfluidic platform that overcomes these barriers by exploiting the ability to perform isothermal PCR via natural convection. We apply “chemical programming” to manipulate the interplay between the PCR biochemistry and the microscale convective flow field, enabling 100% repeatability to be achieved in a format that can be easily and cost-effectively manufactured, dramatically increasing simplicity, portability, and affordability. We demonstrate rapid replication of targets associated with multiple pathogens, including SARS-CoV-2, attaining performance rivaling ultra-fast PCR instruments while using lower reagent concentrations than conventional protocols. This discovery paves the way for widespread adoption of PCR-based analysis at the PON, making it possible to bring lab-quality diagnostics to decentralized settings. |
| 11:45 |  | 基調プレゼンテーション Emerging Trends in Microfluidics Inspired by Nature 自然に想を得たマイクロフルイディクスにおける新たな動向 Anderson Shum, Professor, Department of Mechanical Engineering; Director, Advanced Biomedical Instrumentation Centre, University of Hong Kong, Hong Kong
The field of microfluidics has led to a wide variety of advances in bioanalyses, fluid characterization, materials design and many other applications. Microfluidics is now commonly used as a tool well integrated into the final application. Further development of the field of microfluidics requires new ideas in the design of the microfluidic devices and strategies in general. In this talk, I will discuss a few potential trends in the devices, for instance, by making the devices more responsive to the environment, enhancing the exchange of materials with the micro-environment and extending to a wider range of fluids. The resultant devices enable microfluidics to capture advantages and features that are often observed in Nature, such as in plants, vasculature, and cellular environment. |
| 12:15 | 3D Printed Microfluidic Gadget for Biomedical Daily Grind: From Culturing Tumor Spheroids to Niche Recapitulation 腫瘍スフェロイドの培養からニッチな再現まで、日々繰り返されるバイオメディカルのための3Dプリントマイクロ流体ガジェット Liang Zhao, Associate Professor, Beijing University of Technology, China
Looking back the history of the microfluidics’ development, all the key surging points was initiated from the application of new fabrication modality, such as soft-lithography-based prototyping, which revolutionized this field by greatly lowering the accessibility of microfabrication. We believe the trend for lowering and democratizing the fabrication process should keep going on because biologists begin to harness the microengineering technologies to address the problems during biological experiments such as building and recapitulating organ-like functions on a transparent circuit with highly temporal-spatial controllable way. Herein we present an easy operating microfluidic physiological system that enables liver-tumor spheroids interactions within a 3D printing-cast-based microfluidic chip, recapitulating the liver-tumor microenvironment with an integrative format.
| 12:45 | Networking Luncheon in the Exhibit Hall -- Network with Exhibitors, View Posters and Engage with Colleagues. **Lunch is Japanese Bento** 展示ホールでのネットワーキングランチ -- 出展者や同業者との交流、ポスターの観覧 | | Session Title: Lab-on-a-Chip and Microfluidics -- Current Landscape and Applications Segments セッションタイトル:ラボオンチップとマイクロフルイディクス - 現在の見通しとアプリケーションのセグメント |
| | 14:00 |  | 基調プレゼンテーション RNA Sequencing using a Nanofluidic Device with Dual In-Plane Nanopore Sensors RNAシーケンシングを用いた2重面内型ナノポアのナノ流体デバイス Steve Soper, Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine, The University of Kansas, United States of America
With the development of next generation sequencing (NGS), the field of transcriptomics has seen tremendous advancements opening up opportunities for improved diagnostics of diseases such as cancers and infectious diseases. RNA sequencing enables measurement of single nucleotide variants (SNVs), insertions and deletions, detection of different transcript isoforms, splice variants, and chimeric gene fusions. Although NGS has been useful for unraveling RNA structure and function, several technical difficulties remain including the need for reverse transcription and PCR amplification, which can mask epitranscriptomic modifications. To address NGS issues, we are developing an exciting new sequencing technology called Exonuclease Time-of-Flight (X-ToF) for the label-free detection and identification of single molecules. The hypothesis behind X-ToF is, “individual molecules moving electrokinetically through a 2D nanotube will experience a time-of-flight (ToF) that is dependent upon its molecular identity.” X-ToF is a nanofluidic device comprised of input/output channels, a nano-scale enzymatic bioreactor, and a flight tube equipped with a pair of in-plane nanopores to measure the ToF of a single ribonucleotide monophosphate (rNMP). Each rNMP is produced from an unamplified RNA molecule using a processive exoribonuclease, XRN1. In this presentation we will discuss the high rate manufacturing of the X-ToF chip with sub-5 nm in-plane nanopores using nano-injection molding from a cyclic olefin polymer (COP) plastic. The in-plane pores are situated at either end of a nanochannel (50 x 50 nm; 5 µm long) that generate current transient signals to detect and deduce the identity of rNMPs. The ToF is dependent on the apparent electrophoretic mobility of the molecule. The identity is determined from the ToF, the current transient amplitudes, and dwell times using multi-parameter Principle Component Analysis (PCA). We will show the ability to detect (detection efficiency ~100%) single rNMPs with identification accuracies >98%. Finally, we will discuss the generation of solid-phase nano-bioreactors using XRN1, which can cleave ssRNA in the 5’ to 3’direction releasing rNMPs. Unique properties of the immobilized enzyme will be presented in terms of its processivity and kinetic rate of cleaving single RNA molecules. |
| 14:30 |  | 基調プレゼンテーション Title to be Confirmed. タイトル未定 Valerie Taly, CNRS Research Director, Professor and Group leader Translational Research and Microfluidics, Universite Paris Cite, France
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| 15:00 |  | 基調プレゼンテーション Title to be Confirmed. タイトル未定 Amy Shen, Professor, Okinawa Institute of Science and Technology Graduate University, Japan
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| 15:30 | Mid-Afternoon Coffee and Tea Break and Networking in the Exhibit Hall 休憩および展示ホールでのネットワーキング | 16:00 |  | 基調プレゼンテーション Title to be Confirmed. タイトル未定 Lydia Sohn, Almy C. Maynard and Agnes Offield Maynard Chair in Mechanical Engineering, University of California-Berkeley, United States of America
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| 16:30 |  | 基調プレゼンテーション Modular Design of 3D Printed Microfluidics for Bioprocess Applications バイオプロセス用途向け3Dプリントマイクロフルイディクスのモジュラーデザイン Noah Malmstadt, Professor, Mork Family Dept. of Chemical Engineering & Materials Science, University of Southern California, United States of America
As 3D printing replaces traditional clean room manufacturing for microfluidic engineering applications, it’s becoming clear that this transition offers not only lower cost and faster design iterations, but also new opportunities for fluidic routing and control that are only possible due to the inherent three-dimensional nature of these systems. Over the past several years, we have developed design principles that take advantage of this three-dimensionality, as well as demonstrating several applications that benefit from this approach. At the core of these design principles is the modularity of microfluidic unit operations. In addition to designing prototypical unit operations such as mixers, splitters, flow focusers, droplet generators, thermal and optical sensors, and world-to-chip interfaces, we have developed systematic approaches to combining these modules into microfluidic circuits with predictable behaviors. This approach can be used to rapidly prototype complex microfluidic operations by assembling physically distinct modules as well as to design monolithic microfluidic devices which can be printed in a single run. We have demonstrated the power of this approach by building several micro- and milifluidic systems for bio-analysis and bioprocess applications. These include systems for biomarker diagnostics, automated high-throughput affinity screening, and rapid manufacturing of vaccine lipid nanoparticles. We have also demonstrated how entire systems can be treated as modules, allowing for scaling of bioprocess production lines by massive parallelization. |
| 17:00 |  | 基調プレゼンテーション Lab on a Particle Technology for Functional Discovery of Rare and Unconventional T-Cell Receptors 希少および非従来型T細胞受容体の機能探索のためのラボオン粒子技術 Dino Di Carlo, Armond and Elena Hairapetian Chair in Engineering and Medicine, Professor and Vice Chair of Bioengineering, University of California-Los Angeles, United States of America
Lab on a particle technologies are emerging as a rapidly adopted platform for performing single-cell functional screening leveraging standard instrumentation, such as flow cytometers and microfluidic single-cell sequencing platforms. Each cell and its secreted products can be analyzed and sorted using widely available fluorescence activating cell sorters operating at up to a 1000 cells per second, promising to democratize single-cell technologies. The platform enables sorting cells based on secreted products for the discovery of antibodies, the development of cell lines producing recombinant products, and the selection of functional cells for cell therapies. Here, I will describe our progress in using nanovial particles for the characterization and discovery of antigen-specific and metabolite-specific T cells. Nanovials enable selective binding, functional characterization of secretion of cytokines and other effector molecules, and sorting of T cells for downstream T-cell receptor sequencing and functional annotation. We apply oligo barcoding technology to both multiplex target antigens and functional performance and link this to the TCR sequence information. Nanovials can enable more rapid discovery of cancer-specific TCRs and TCRs against metabolites presented by unconventional MHC-like molecules, with high accuracy, promising to transform the discovery of critical recognition elements for improved T cell therapies. |
| 17:30 | Networking Reception in the Exhibit Hall with Japanese Beer and Sake 展示ホールでのネットワーキングレセプション | 18:30 | Close of Day 1 Conference Programming 1日目カンファレンスプログラム終了 |
2023年10月6日(金)08:00 | Morning Coffee, Tea and Networking in the Exhibit Hall 展示ホールでのネットワーキング | 09:00 |  | 基調プレゼンテーション Rapid and Signal Crowdedness-Robust In-Situ Sequencing through Hybrid Block Coding ブロック符号化のハイブリッドによる迅速かつ信号の混雑にロバストなIn-Situシーケンシング Yanyi Huang, Professor, Peking University, China
Spatial transcriptomics technology has revolutionized our understanding of cell types and tissue organization, opening new possibilities for researchers to explore transcript distributions at subcellular levels. However, existing methods have limitations in resolution, sensitivity, or speed. To overcome these challenges, we introduce SPRINTseq (Spatially Resolved and signal-diluted Next-generation Targeted sequencing), an innovative in situ sequencing strategy that combines hybrid block coding and molecular dilution strategies. Our method enables fast and sensitive high-resolution data acquisition, as demonstrated by recovering over 142 million transcripts from 453,843 cells from four mouse brain coronal slices in less than two days. Using this advanced technology, we uncover the cellular and subcellular molecular architecture of Alzheimer's disease, providing unprecedented insights into abnormal cellular behaviors and their subcellular mRNA distribution. This improved spatial transcriptomics technology holds great promise for exploring complex biological processes and disease mechanisms. |
| 09:30 |  | 基調プレゼンテーション Analytical Assays on Paper Platforms: As Simple as Possible ペーパープラットフォーム上の分析アッセイ:可能な限りシンプルに Daniel Citterio, Professor, Keio University, Japan
There is a continuously growing demand for analytical assays that can be performed on-site by minimally trained operators without the need for sophisticated laboratory equipment and reagent handling. In this context, (microfluidic) paper-based analytical devices (µ)PADs, including lateral-flow immunoassays (LFIAs), have gained significant attention, particularly for point-of-care testing in medical diagnostics. Over the past few years, we have demonstrated that certain analytical assays conventionally depending on benchtop instruments can be implemented into simple paper platforms not requiring any reagent handling. Examples include paper-based devices for the naked eye “calibration-free” colorimetric semiquantitative analysis of clinically relevant parameters that can be operated by minimally trained users with minimal risk of incorrect result misinterpretation. The presentation will also introduce one of our latest efforts towards boosting assay sensitivity on paper by adapting the CRISPR/Cas system to a paper-based analytical platform in the form of a simple, rapid, and highly sensitive detection method for non-nucleic acid targets by integrating CRISPR/Cas12a and an enzyme-linked immuno-sorbent assay (ELISA). |
| 10:00 | Technology Spotlight Presentation 注目技術のプレゼンテーション | 10:30 | Mid-Morning Coffee and Tea Break and Networking in the Exhibit Hall 休憩および展示ホールでのネットワーキング | 11:00 | Technology Spotlight Presentation 注目技術のプレゼンテーション | 11:30 | Technology Spotlight Presentation 注目技術のプレゼンテーション | 12:00 | Networking Luncheon in the Exhibit Hall -- Network with Exhibitors, View Posters and Engage with Colleagues. **Lunch is Japanese Bento** 展示ホールでのネットワーキングランチ -- 出展者や同業者との交流、ポスターの観覧 | | Session Title: The Deployment of Microfluidics for Developing Microphysiological Systems (MPS), Organs-on-Chips セッションタイトル:開発中の生体模倣システム、臓器チップのためのマイクロフルイディクスの展開 |
| | 13:30 | Initiatives of AMED-MPS Projects for Industrial Implementation of MPS in Japan and Collaboration with Asia 日本におけるMPSの産業化とアジアとの連携に向けたAMED-MPSプロジェクトのイニシアチブ Seiichi Ishida, Guest Researcher, National Institute of Health Sciences, Professor, Sojo University, Japan
AMED-MPS2 project started in 2022 and has been leading the initiative for the implementation of MPS originated in Japan to industries. Due to the unique nature of the culture environment of MPS, it has become clear that there are "points to consider" that differ from conventional culture methods in order to maintain healthy cell function,e.g. cell functionality, cell adhesion property, and medium perfusion conditions. Our efforts how to deal with such requirements for MPS industrial implementation will be introduced. Beyond these efforts will be the regulatory acceptance of the data obtained by MPS. Discussions what MPS should be as the test methods for regulatory usage based on the considerations will be presented, which are taking place in AMED MPS-RS (RS stands for “regulatory science”). MPS-RS has launched MPS Consortium for Industrial Implementation and Regulatory Acceptance, which gives the discussion table among four stakeholders, academia, supplier, end user, and regulator. The MPS Consortium are also collaborating with iMPSS, founded in this June, and its Asia-Pacific regional chapter. These activities will be presented. | 14:00 | MPS Platforms with High Operability for Commercialization 商業化に向け高い操作性を備えたMPSプラットフォーム Hiroshi Kimura, Professor, Micro/Nano Technology Center, Tokai University, Japan
Microphysiological systems (MPSs) have been widely studied as a novel
method for estimating the effects and toxicities of drugs, providing an
alternative to animal tests in drug discovery. In EU and USA, various
types of MPS are commercially available by some companies, and more
recently, their practical application has been well promoted. Although
MPS has been actively researched in Japan, there has been almost no
practical MPS to date. Japan Agency for Medical Research and Development
(AMED) has conducted an MPS development project with the aim of
commercializing domestically produced MPS since 2017. Our research group
has developed two types of MPS, Fluid3D-X and Kinetic pump Integrated
Microfluidic Plate, for commercialization in collaboration with Japanese
manufacturing companies in the project. Our proposed MPSs are expected
to facilitate high quality cell-based assays in drug discovery and
biology due to their ease of use and high throughput. In this
presentation, I present the overview of these MPS’s functions and
examples of the drug evaluation studies using the MPSs. | 14:30 | The NIH Microphyiological Systems Program: In Vitro 3D Models for Safety and Efficacy Studies NIH生体模倣システムプログラム: 安全性と有効性研究のためのIn Vitro 3Dモデル Danilo Tagle, Director, Office of Special Initiatives, National Center for Advancing Translational Sciences at the NIH (NCATS), United States of America
Approximately 30% of drugs have failed in human clinical trials due to
adverse reactions despite promising pre-clinical studies, and another
60% fail due to lack of efficacy. A number of these failures can be
attributed to poor predictability of human response from animal and 2D
in vitro models currently being used in drug development. To address
this challenges in drug development, the NIH Tissue Chips or
Microphysiological Systems program is developing alternative innovative
approaches for more predictive readouts of toxicity or efficacy of
candidate drugs. Tissue chips are bioengineered 3D microfluidic
platforms utilizing chip technology and human-derived cells and tissues
that are intended to mimic tissue cytoarchitecture and functional units
of human organs and systems. In addition to drug development, these
microfabricated devices are useful for modeling human diseases, and for
studies in precision medicine and environment exposures. Presentation
will elaborate in the development and utility of microphysiologicals
sytems and in the partnerships with various stakeholders for its
implementation. | 15:00 | Microphysiological Systems (MPS) With Perfusable Vascular Network for Pharmacological and Organogenesis Applications 薬理学的および器官形成用途のための灌流可能な血管ネットワークの生体模倣システム Ryuji Yokokawa, Professor, Department of Micro Engineering, Kyoto University, Japan
Microfluidic devices have been used to answer scientific questions in
many lifescience research fields. Microphysiological systems (MPS)
mimics the functions of human biological organs and can be used to
measure physiological functions that are difficult to measure on a
culture dish. We have employed two approaches to create the interface
between organ cells and vascular networks: a two-dimensional method in
which organ cells and vascular endothelial cells are co-cultured on a
porous membrane such as Transwell (2D-MPS), and a three-dimensional
method in which the spontaneous patterning ability of vascular
endothelial cells is utilized (3D-MPS). As an example of 2D-MPS, we
developed a renal proximal tubule model and a glomerular filtration
barrier model using iPSC-derived organoid cells, which enables us to
evaluate reabsorption, filtration, and nephrotoxicity. For 3D-MPS,
angiogenesis and/or vasculogenesis are utilized to anastomose a
fibroblast spheroid and tumor spheroids to create tumor
microenvironments to evaluate the efficacy of an anti-tumor drug under a
flow condition. We also developed an on-chip vascular bed to co-culture
with any kind of tissues that do not have enough angiogenic factors to
induce angiogenesis. It was applied to kidney and brain organoids for
evaluating the effect of vessels on their development. The vascular bed
chip enabled to culture a kidney organoid at the air-liquid interface
(ALI) that is required for nephrogenesis and to separately supply two
media for the organoid and vascular bed. Proposed assay platforms will
further contribute to realize pharmacological applications and to
understand in vivo organogenesis. We keep exploring how micro/nano
fabrications can deepen science at the interface between blood vessels
and organs. | 15:30 | Late-Afternoon Coffee, Tea and Networking in the Exhibit Hall 展示ホールでのネットワーキング |
* 不測の事態により、事前の予告なしにプログラムが変更される場合があります。
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