BIODETECTION TECHNOLOGIES 2014 - 生物学的検出技術学会、2014年 -
2014年6月10 - 11日 米国、メリーランド州、ボルチモア

           
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今度で22回目となるBIODETECTION TECHNOLOGIES 2014は、Detection Technologiesシリーズの学会であり、生物学的な脅威の検出と識別およびポイントオブケア分析手法の専門家が一堂に会するイベントとして国際的にも高い評価を得ています。会期中は多彩なセッションが予定されており、生物学的防衛技術や生物医学技術のエンドユーザーから得られたフィードバックの分析、この分野の研究開発や商業化に向けた取り組みの最新動向などが紹介されます。

 
•    高速バイオセンシング、生物学的検出および分析
•    複数の脅威や病原体の同時検出
•    宿主反応検出のためのバイオシグネチャの発見と分析
•    病原体/ウィルス/脅威の検出と識別に対応するポイントオブケア/臨床応用技術
•    リソースが限られた検査室や臨床現場、モバイルラボに対応するポイントオブケアアッセイ
•    マイクロアレイとシーケンシング技術の進歩
•    現場で導入可能な機器:携帯性/互換性/信頼性/拡張性
•    非PCRおよびPCRベースの検出技術
•    検出感度/検査/評価/検証の問題
•    動物と植物の病原体
•    バイオエレクトロニクス
•    DNAチップ、核酸センサー、アプタセンサー
•    酵素ベースのバイオセンサー
•    免疫センサー
•    ラブオンチップ
•    マイクロ流体工学と固定化技術
•    天然および合成受容体(MIPを含む)
•    有機体および全細胞ベースのバイオセンサー
•    プロテオミクス、単一細胞解析、がん細胞検出
•    信号伝達技術(磁気、圧電、光学、直接電気化学)
 
 
 
 
 
 
 
メディアスポンサーと学会パートナー
 
 
 
 
 
2014年6月10日(火)
 
8:00        登録手続き、展示会見学/ポスター発表の設営、コーヒーと軽食
 
生物学的標的識別のための遺伝子増幅
 
9:00       病原体識別強化とオープンアレイプラットフォームを利用した献血者試料のマルチプレックス検査
 
Robert Duncan, PhD, Staff Scientist, FDA Center for Biologics Evaluation and Research, U.S. Food and Drug Administration
 
Detection of pathogens in blood is required for donor screening and diagnostics. We recently demonstrated effective multiplex screening for 9 pathogens simultaneously with the OpenArray nanofluidic real-time PCR platform. The blood borne pathogen OpenArray platform has been expanded to screen 26 pathogens with discrimination to the species, strain or genotype level. High sensitivity of detection was demonstrated with 92 blood donor specimens.
 
9:30        治療の指針となるバークホルデリア属細菌の検出
 
R. Paul Schaudies, PhD, CEO, GenArraytion, Inc.
 
In collaboration with USAMRIID, thirty isolates each of Burkholderia pseudomallei and mallei were screened using a genotyping microarray. Results were combined with antibiotic sensitivities to generate multiplexed real-time PCR to identify and characterize these organisms.
 
10:00    導電性DNAによる複数標的のリアルタイム同時検出
 
Fred Albert, PhD, President, Bridger Technologies, Inc.
 
While progress is being made on the rapid and simultaneous detection of multiple targets, evolutionary improvements to conventional biodetection systems are unlikely to overcome their inherent limitations. A breakthrough technology that enables the simultaneous detection of multiple targets in less than one minute will be presented. This non-PCR, conductive DNA-based technology has been shown to be highly sensitive, highly specific, and deliver accurate results even in the presence of background and contaminants that debilitate or foul other detection systems.
 
10:30    休憩、展示会/ポスター発表の見学
 
11:00    環境調査に対応する完全自律型の生物学的検出システム
 
Mark Burton, Northrop Grumman Corporation
 
The Next Generation Automated Detection System (NG-ADS) Biodetector that continuously collects and analyzes air samples to detect and identify biological threat agents will be presented.  The biodetector operates autonomously between routine consumables replenishment, and can be operated 24/7 year round. The detector is designed to use a multiplexed PCR assay to reliably detect threat agents simultaneously with high sensitivity, low limit of detection, and an extremely low false positive rate.  Samples are archived for further analysis if desired.  These systems have recently participated in a field test to demonstrate performance in an operational environment.
 
11:30    新たな診断戦略に基づく臨床上の困難な問題への対応
 
Harshini Mukundan, PhD, Research Scientist, Los Alamos National Laboratory
 
We will discuss advanced and integrated biodiagnostic strategies at LANL, spanning from rapid biomarker detection to advanced sequencing based analysis, to understand the circulation, emergence and occurrence of drug resistance in a pediatric population in rural Kenya. In addition, we will also talk about novel detection strategies used for the detection of bacterimia, including active tuberculosis, for the first time in this population.
 
12:00    性感染症関連市場に対応するポイントオブケア検査開発の実現
 
Joany Jackman, PhD, Investigator, Center for Point of Care Tests for STD, Johns Hopkins University School of Medicine*
 
The mission of the Johns Hopkins University Center for Point of Care Tests for Sexually Transmitted Diseases (JHUC) is to provide expertise, guidance and samples to enable the development of the best available test platforms for diagnosis of sexually transmitted infections (STIs). To that end JHUC has conducted focus groups, facilitated meetings and other studies to determine the most important attributes of successful test for STIs in a variety of point of care settings.  These data and their relevance to the global market for POCT for STIs will be presented.*In collaboration with: M.Jett-Goheen, A.Rompalo, T.Hogan, C.Gaydos
 
12:30    午前のセッション終了
 
生物学的標的の新たな検出戦略
 
2:00        直接多重化ウィルス検出
 
John H. Connor, PhD, Professor, Dept of Microbiology, Boston University School of Medicine
 
We have developed an LED-based virus detection technology that is label-free and multiplexed. This technology allows the identification of viruses that cause hemorrhagic fever without the need for nucleotide isolation and amplification on a rapid time-scale in platform that can be used at the point of care.
 
2:30        類鼻疸の高速診断に対応する側方流動イムノアッセイ(LFI)の最適化
 
David AuCoin, PhD, Research Assistant Professor, Dept Microbiology and Immunology, University of Nevada School of Medicine
 
Burkholderia pseudomallei is a soil-dwelling bacterium that is the causative agent of melioidosis.  Laboratory detection of B. pseudomallei is difficult and slow, because of challenges with culturing and a lack of validated diagnostic reagents, but this has been the best approach for diagnosis of melioidosis.  Our goal, therefore, has been to develop a rapid point-of-care immunoassay for the diagnosis of melioidosis.  Our initial efforts have focused on developing a CPS-specific monoclonal antibody (mAb).  The same mAb was used to produce a prototype lateral flow immunoassay (LFI) that is capable of detecting CPS in a variety of patient samples. The CDC is currently testing the LFI against a large panel of B. pseudomallei, related and near neighbor strains.  Results of these tests and plans for further field testing will be reported.
 
3:00        PCRフリーな視覚的DNA検出技術の開発に向けた取り組み
 
Mahesh Uttamchandani, PhD, Assistant Professor, DSO National Laboratories, National University of Singapore, Singapore
 
Novel methods to detect DNA sequence specifically through color change reactions will be described. To transduce molecular recognition events into visual readouts, we have engineered assays which have exploited split DNAzymes and gold nanoparticles. The G-quadruplex DNAzymes were successfully applied to the detection of Salmonella and Mycobacterium DNA, as well as in genotyping a single base difference from within human genomic DNA samples.  An integrated workflow was capable of detecting DNA samples through a color change within just 5-15 minutes. The gold nanoparticle method offered much greater sensitivity, lowering the limit of detection visually, without the need for PCR amplification.
 
3:30        休憩、展示会見学
 
4:00        ポイントオブケアへの応用に焦点を絞り込んだ磁気流体力学技術
 
Christian Reis, Group Lead - Biotechnology Processes, Fraunhofer IPA, Germany
 
Magnetic bead handling is a common tool in on-chip biodetection systems and research is improving fast.  A technology for detecting the load of a magnetic particle by forcing the particle to describe certain trajectories with switching magnets will be presented. This allows us to net-focus magnetic beads in a hydrodynamic system and to provide quantitative insight for the number of molecules bound to the particle surface.
 
4:30       クラウドマッピングと分光レーザー誘起蛍光(LIF)分類を可能にするLIDAR技術を利用したバイオエアロゾルスタンドオフ検出
 
Sylvie Buteau, PhD, Scientist, Defence Research and Development Canada, Canada*
 
A standoff sensor called BioSense was developed to demonstrate the capacity to map, track and classify bioaerosol clouds from a distant range and over wide area.  The concept of the system is based on a two steps dynamic surveillance: 1) cloud detection using an infrared (IR) scanning cloud mapper and 2) cloud classification based on a staring UV Laser Induced Fluorescence (LIF) interrogation. The main challenge is classification, which relies on a spectrally resolved UV LIF signature library. The system showed good performances even prior to further optimization. *In collaboration with: J.-R.Simard, G.Roy, P.Lahaie

5:00        iTIRF−分子診断に対応する携帯電話ベースのバイオセンサー
 
Alexander N. Asanov, PhD, President and CSO, TIRF Labs, Inc
 
A novel molecular diagnostics technology based on Total Internal Reflection Fluorescence, termed - iTIRF, will be presented.   iTIRF is capable of simultaneously detecting proteins, nucleic acids, and metabolite biomarkers.  iTIRF microarrays employ silk fibroin, which allows for much greater immobilization of reagents and a resulting signal that is a thousand-fold greater than that with classical TIRF.  Additional advantages of the biosensor, and plans for further development, will also be described.
 
5:30        病原菌の追跡と識別に対応するゲノムベースのアプローチ
 
Willy Valdivia-Granda, PhD, CEO, Orion Integrated Biosciences Inc.
 
The microbiome of an animal contains approximately 10 times the number bacterial cells than host cells and around 150 times more genes.  Using a library of motif fingerprints and genomic signatures for pathogens of biodefense and agrodefense relevance, we performed an extensive survey of the metagenomic samples of humans and domestic animals.  We have used our motif fingerprint scanning technology to perform inclusion/exclusion bioforensic and attribution analysis.  The implications of our work in biosurveillance and standardized nucleic acid- or antibody-based detection system development will be discussed.
 
6:00        出展企業/スポンサー企業のプレゼンテーション−I/締めくくりの討論
 
6:15        展示会ホールでの歓迎レセプションとポスター発表の見学
 
 
 
2014年6月11日(水)
 
8:00        展示会/ポスター発表の見学、コーヒーと軽食
 
化学物質と毒素の検出
 
9:00        環境試料での活性リシン検査のための新規細胞ベースアッセイ
 
Baolin Zhang, PhD, Senior Investigator, Division of Therapeutic Proteins, Office of Biotechnology Products, Food and Drug Administration
 
Ricin is a deadly protein toxin with potential use as a bioterror agent. The threat of ricin attack has increased over the past decade and it has been linked to over a dozen criminal cases. The purpose of this project is to advance the current science of detection of active ricin based on its binding to the cell surface by functional ricin Chain B, cellular uptake of the catalytic Chain A, and subsequent cell death. The ability to discriminate between active and 'dead' ricin forms with the cell-based analysis can provide additional information on the risk factors associated with the sample.
 
9:30        光学バイオセンシングシステムをベースにしたミクロシスチン-LRの自動オンラインリアルタイム検出
 
Han-Chang Shi, PhD, Professor, School of Environment, Tsinghua University, PR China
 
To minimize the health risks to the public, cyanotoxin detection methods that are rapid, sensitive, real time, and high frequency must be established.  An novel automated optical biosensing system (AOBS) was developed for the rapid detection of microcystin-LR (MC-LR). Results using an indirect competitive detection mode will be presented.   The quantification of MCLR ranges from 0.2 to 4 µg/L, with a detection limit determined as 0.09 µg/L.
 
10:00    マイクロカンチレバーが実現する生物学的検出
 
Rick Venedam, PhD, Senior Scientist, National Security Technologies, LLC
 
Embedded piezoresistive microcantilever (EPM) sensors have been used in the detection of a variety of analyte species.  EPM sensors utilize a tiny piezoresistive microcantilever partially embedded into a sensing material to produce a sensing element that is compact, simple, resistant to movement and shock, and suitable for remote sensing applications. In this project  we used sensing materials consisting of an immobilizing polymer functionalized with either target enzymes or antibodies to detect two biological agents, Bacillus subtilis and Diisopropyl fluorophosphates, a simulant for organophosphate nerve agents.  Sensing results are presented for both types of EPM sensors.
 
10:30    休憩、展示会/ポスター発表の見学
 
11:00    フォトニックノーズ:多用途の単純な検出ツール
 
Leonardo Bonifacio, PhD, Research Scientist, Opalux Inc., Canada
 
The Photonic Nose platform, which is the first platform to make use of photonic crystals as an artificial nose, will be presented. It is a simple, cost-effective and versatile chemical sensing platform applicable for the detection of various analytes both in gas and liquid phases.  Most current nose technology is based on relatively complex and costly platforms.  The photonic nose can be used for analysis of both liquid and vapour phase samples, and is based on arrays of specially designed photonic sensors.  The combinatorial response can be analyzed by use of simple digital cameras for remote and near instantaneous verification.  We have implemented proof-of-concept projects that addressed challenges in areas that include bacterial detection and identification, safety testing for food, beverages, water and crude oil quality, and a number of other applications.
 
11:30    MEMSベースの新たなマイクロコリオリ濃度測定技術
 
Mike Touzin, Endress+Hauser Flowtec, Switzerland
 
Laboratory and field hazardous area applications of a novel MEMS technology for density measurement will be presented.  This microfluidic sensor, based on the Coriolis principle, can measure density/specific gravity, temperature and viscosity.  These very compact MEMS devices are immune to vehicular vibration and have an extremely fast response time, due their high resonant frequencies.  The ability to differentiate between types of fuels such as gasoline, ethanol, methanol, diesel, biodiesel, butanol, and to detect water and air contamination using density measurement, will be demonstrated. Concentrations of fuel blends such as E85 and others can be accurately determined.
 
12:00    分子認識に対応する中赤外量子カスケード(QC)レーザー
 
Mariano Troccoli, PhD, Director of Product Development, AdTech Optics, Inc.
 
Recent results with high performance mid-infrared quantum cascade lasers both for high power and single-mode operation will be presented.  In addition, their applications to molecular recognition will be described and results on multi-wavelength detection of important chemical compounds in a single multi-laser system are detailed.
 
12:30      午前のセッション終了
 
2:00        免疫シグネチャによる宿主反応ベースの生物学的検出
 
Stephen Albert Johnston, PhD, Co-Director, Center for Innovations in Medicine, Professor, School of Life Sciences, Biodesign Institute, Arizona State University
 
Most of biodetection efforts have focused on sensing the pathogen. This has serious basic and practical limitations. A simple technology based on immunosignatues, for detecting host changes in response to pathogens, will be presented.  It is very sensitive and inexpensive.  It is commercializable and importantly would enable new levels of biosecurity as a by product of standard clinical practice.
 
検出のためのツール−マイクロ流体と酵素
 
2:30        開発時間を短縮し、リスクを低減するために開発されたマイクロ流体コンポーネントのツールボックス
 
Leanna Levine, PhD, CEO, Aline, Inc.
 
A toolbox of engineered microfluidic components, including metering channels, valves, vents, pumps, and de-bubbling, can be engineered into any number of desired footprints.  Optimized actuation inputs and protocols, and design specifications ensure well characterized and repeatable performance.  Through choice of materials and design constraints, we demonstrate data on the repeatable performance of a device that meters, mixes, debubbles and dispenses. Data is presented on component reproducibility and scalable production.

3:00        SoundStream−可搬型高速診断装置に対応するマイクロ流体ベースのアッセイプラットフォーム
 
Arlene Doria, PhD, CEO, DEFINEQA Inc.
 
An innovative microfluidic technology known as SoundStream, will be described.  The field of microfluidics is plagued with challenges in integration, fluid control, and limited sample preparation strategies.  SoundStream employs the use of oscillating microbubbles to perform multiple assay steps including pumping, mixing, bead assay detection, plasma/serum separation, cell lysis, and particle size separation.  The technology is easy to integrate with bioassay detection methods.  It reduces the complexity of the microchip design and is scalable. Finally, the platform can be powered by simple batteries for rapid portable diagnostics.
 
3:30        休憩、展示会/ポスター発表の見学
 
4:00        原核生物DNAの特異的な濃縮のための組換えトポイソメラーゼの応用
 
Natalia Sandetskaya, PhD, Fraunhofer Institute for Cell Therapy and Immunology IZI, Germany
 
The development and application of the novel molecular tool for the targeted enrichment of prokaryotic DNA in complex samples will be presented.  The DNA binding subunit of the bacterial topoisomerase II, gyrase, was expressed, purified and immobilized on magnetic particles. Results showing specific affinity towards bacterial DNA in the samples with high background of eukaryotic DNA will be described. This method is a promising approach for the preparation of such type of the samples, for example, in molecular diagnostics of sepsis.
 
4:30        熱安定性の高い新規ウィルスDNAポリメラーゼが促すDNAおよびRNA標的のポイントオブケア分子検出
 
Hemanth Shenoi, PhD, Lucigen Corp
 
Point of care (POC) molecular detection of pathogens requires improvements in enzymes, formulation and stability. OmniAmp enzyme is a novel isothermal amplification polymerase for loop-mediated amplification (LAMP) amplification of RNA or DNA.  The unique inherent reverse transcriptase activity of the enzyme allows single enzyme detection of RNA targets.  OmniAmp can be formulated dry for ambient storage and transport. Detection of amplification products can be accomplished using multiple methods.
 
5:00        出展企業/スポンサー企業のプレゼンテーション−II/優れたポスター発表の紹介
 
5:30        締めくくりの言葉、学会終了

* 不測の事態により、事前の予告なしにプログラムが変更される場合があります。

 

 
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