7th International Conference Sample Prep 2013 - 第7回国際試料調製学会、2013年 -
2013年5月9 - 10日 米国、カリフォルニア州、サンディエゴ、ヒルトンサンディエゴリゾート

 
         
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SAMPLE PREP 2013は、Knowledge Foundationが各地で開催しているSample Prepカンファレンスシリーズの1つであり、試料調製技術の専門家を対象とした国際的にも評価の高い学会です。生物医学、生物学、化学などの分野の薬剤や物質、脅威を、ポイントオブケアや研究室、各種の現場環境で検出、同定、診断、分析するための試料調整技術に焦点を絞り込んだ学会であり、研究開発の最新の成果、市場投入間近の技術、各種の用途などが紹介されることになっています。今年の学会では、以下のようなトピックが取り上げられる予定です。

- 試料収集、(事前)濃縮、溶解、標的抽出のための手堅い手法

- 早期診断における試料調製の重要な役割

- 試料調製からシーケンス解析までの作業を迅速に行なう方法

- ポイントオブケアでのサンプリング、検出、分析

- マイクロ流体工学を利用した試料調製

- オンチップ試料調製

- ロバストサンプリング法(試料調製における自動ハイスループットコンビナトリアルアプローチ)

- 高感度な試料の調整に対応する各種の増幅法

- 別システムおよび統合モジュールのアプローチによる試料調製

- 生物検知とサンプリングの技術やデバイスを導入する可能性の高いエンドユーザー

- 試料調製と各種応用技術に対する代替アプローチと革新的なアプローチ

- 現場で使用可能な装置−互換性/信頼性/拡張性

 

この学会では、生物兵器への防衛、現場やポイントオブケアでの生物医学的応用や臨床応用、食品や水の検査、環境や農業分野でのサンプリングなどに利用可能なロバストサンプリングや生物法医学の新たな手法も紹介される予定です。会期中は、行政機関や大学、企業などに所属するトップクラスの専門家たちが登場し、技術開発や実用化の焦点となっている以下のような問題や領域をめぐって議論を展開します。

- 標的の濃縮とバックグラウンドデプリーションの手法

- 細胞および分子レベルでの希薄な物体の分離

- 不混和相の精製

- 強固な(ろ過できない)基質からの抽出を可能にする新たな手法

- 次世代シーケンシングのための核酸およびタンパク質ベースの試料調製

- 試料調製に対応するナノテクノロジーと微細化の課題

- 試料調製、検出、分析のための新たなアッセイとシーケンシング技術

- 各種用途での試料調製における標準化と規制の問題

- 検出/診断と医薬品に対応する試料調整技術


 
 
 

メディアスポンサーおよびカンファレンスパートナー

 
 
2013年5月9日(木)
 
8:00        登録手続き、展示会見学/ポスター発表の準備、コーヒー
 
8:50        主催者代表による歓迎と開会の挨拶
 
9:00        次世代バイオセンサーの開発が各種の試料調製アプローチに及ぼす影響
Charles Young, PhD, Principle Professional Staff, Asymetric Operations Dept, The Johns Hopkins University Applied Physics Laboratory; Assistant Research Professor, Dept of Geography and Environmental Engineering, The Johns Hopkins University
Biological sensors represent one facet of a system designed to identify a biological agent. In addition to the sensor, a detection system must account for sample acquisition, sample storage/transport, sample preparation, assay design/performance and data management. Therefore, a systems approach is critical for the development of next generation sensors since changes to one component can impact the entire system. Over the past 5 years, researchers at JHU/APL have been tracking biosensor development and building a database of commercial-off-the-shelf systems and their capabilities. In this presentation we will discuss the database, current sensor trends and their implication for sample preparation.
 
9:30        ポイントオブニード診断のための試料調製アプローチ
Richard Allen, PhD, Senior Scientist, Biodefense and Food Safety, Luminex Corporation
Complex biological states such as transmissible infectious disease involve a dynamic relationship between pathogenic organisms and their hosts. Accurate assessment of this relationship presents a unique instrumentation and assay challenge. To provide a more comprehensive snapshot of an evolving infection, efforts at Luminex Corporation include development of an automated, field portable diagnostic system based on the open architecture xMAP technology. A key aspect of this system is the ability to test for both the pathogen itself, through molecular techniques, and also host biomarkers that signal the course of infection via affinity capture reagents. Taken together, these data will yield a more accurate and timely treatment, leading to better clinical outcomes than either test alone. The challenge in developing such a system is to devise sample preparation protocols that 1) are versatile enough to encompass either technique, 2) can be deployed onto an automated, inexpensive disposable cartridge, and 3) are rapid enough to both inform clinical decisions and provide multiple measurements throughout disease progression. Luminex will provide an update on sample preparation techniques being investigated for this effort and provide data from initial testing.
 
10:00    試料配送システムの考え方
John C. Carrano, PhD, President and CEO, Paratus Diagnostics, LLC
In this paper we posit that in order to realize pragmatic point-of-care medical diagnostic devices capable of meeting the rigorous requirements of CLIA waiver, that one must solve the inherent “impedance mismatch” between the clinical acquisition of a human patient sample and its delivery to the POC Dx system or device. We work from the assumption that specimen acquisition from the patient must follow standard clinical practice, and that the POC system (e.g. “device”) will be designed to follow a yet to be determined international interface standard (similar in principle to the now ubiquitous Luer-Lock standard). We will present a design concept for a “specimen delivery system” and the associated proposed interface standard, along with experimental data from preliminary rapid prototype models.
 
10:30    休憩時間、展示会/ポスター発表の見学
 
11:00    リアルタイムメタゲノミクス:サンゴ研究から臨床応用まで
Forest L. Rohwer, PhD, Professor of Biology, San Diego State University
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
11:30    病原体生死判定ポリメラーゼ連鎖反応(PCR)法に対応する技術
Sarah Fakih, PhD, Senior Scientist, Food Safety Testing R&D, QIAGEN GmbH, Germany
Nucleic acid detection methods, such as real-time PCR, provide fast and powerful tools to analyze samples for the presence of potentially harmful microbes, but also hold the risk of false positives by detecting nucleic acid from harmless dead cells. We addressed this by a universally applicable tool box for an advanced viability PCR, which centers on the DNA-masking compound Propidium monoazide (PMA), suppressing amplification signals from dead cell DNA. We have pre-developed viability PCR as a complete standardized system to be used with a new illumination device designed to catalyze the PMA reaction. The new tool box system, the illumination device and its function together in viability PCR applications are presented with multiple sets of user data.
 
12:00    ポイントオブケア環境に対応する堅牢な自己完結型試料調整カートリッジ
Season Wong, PhD, Co-Founder and Director, AI Biosciences, Inc.
This presentation will cover the development of AI Biosciences Inc.’s Sample Preparation Cartridge (SPC) which delivers high quality nucleic acids for point-of-care molecular analysis. Our SPC can be operated manually or hands-free using battery pack. The SPC is a closed-system that eliminates cross-contamination and provides hassle-free waste disposal. We will demonstrate its utilities using a wide range of samples. The detection of the derived nucleic acid in a non-laboratory setting will also be discussed.
 
12:30    Knowledge Foundation会員プログラム提供の昼食会
 
2:00        慢性リンパ性白血病(CLL)患者血液からの循環細胞フリーDNAの分離と検出−「試料調製からシーケンス解析までの作業の迅速化」
Michael J. Heller, PhD, Professor, Depts of Bioengineering and Nanoengineering, University of California San Diego*
We have developed a unique sample to answer dielectrophoretic (DEP) technology for rapid isolation and detection of cancer related cell free circulating DNA (cfc-DNA) biomarkers, bacteria and virus directly form blood and other complex biological samples. Using this DEP microarray device, cfc-DNA from chronic lymphocytic leukemia (CLL) patients could be isolated directly from 50uls of unprocessed whole blood in about 15 minutes. The cfc-DNA was then eluted from the DEP chip, PCR amplified using CLL specific primers and sequenced to determine CLL patient specific polymorphisms and mutations. Overall, the use of DEP devices for the rapid isolation of cfc-DNA from a small volume of blood will allow “noninvasive liquid biopsy” point of care (POC) detection and diagnosis of incipient, residual, and recurrent cancer and other diseases.
*In collaboration with: Jennifer Marciniak and Avery Sonnenberg
 
2:30        環境中の生物学的因子を検出するための一般的な試料調製アプローチ
Martin McDonnell, PhD, Principal Scientist, Detection Department, Defense Science and Technology Laboratory - DSTL Porton Down, United Kingdom
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
3:00        血液から細菌の試料を迅速に調整する方法
Alexis Sauer-Budge, PhD, Senior Research Scientist, Fraunhofer Center for Manufacturing Innovation, Fraunhofer USA
Traditionally, bacterial pathogens in the blood have been identified using culture-based methods that can take several days to obtain results. This can lead to physicians making treatment decisions based on an incomplete diagnosis contributing to patient morbidity. To decrease diagnosis time, we are developing a novel sample preparation device for isolating and concentrating dilute bacteria from blood. The device is designed to be a single-use disposable that can be used manually or with a fully automated instrument.
 
3:30        休憩時間、展示会/ポスター発表の見学
 
4:00        ラマン分光法により水の微生物汚染を試薬なしで迅速に同定する方法
Gregory Auner, PhD, Professor, Electrical and Computer Engineering, SSIM Director, Wayne State University
We are developing a reliable and rapid method to assess microbial contamination in water. Raman Spectroscopy is a reagentless, non-destructive, technique that provides a unique spectral fingerprint of bacteria without sample preparation and is conducive to field application since it can provide both qualitative and quantitative analysis. We will present Raman identification of E. coli, Salmonella, Rahnella aquatilis, Enterobacter, Vibrio fluvialis, Pseudomonas, Bacillus subtilis, Listeria, Staphylococcus, and Streptococcus.
 
4:30        デンドリマーにより修飾された多層カーボンナノチューブをベースにしたヒト細胞プリオンのラベルフリー電気化学アプタセンサー
Hafsa Korri-Youssoufi, PhD, Researcher, Institut de Chimie Moléculaire et des Matériaux d’Orsay – ICMMO, University Paris-Sud, France
The present work aims to develop an electrochemical aptamer based biosensor able to detect human cellular prions PrPC as a model biomarker of prion disease with high sensitivity. We designed biosensor using multiwalled carbon nanotubes (MWCNTs) modified with polyamidoamine dendrimers (PAMAM) of fourth generation (G4) able by their amino group to attach ferrocenyl redox marker and the DNA aptamer as bioreceptor. MWCNTs, thanks to their nanostructure organization and electrical properties, allow the distribution of aptamer and redox markers over the electrode surface. We demonstrated that the interaction between aptamer and prion protein leads to variation in electrochemical signal of the ferrocenyl group. High sensitivity detection limit of 0.5 pM and wide linear range of detection from 1 pM to 10 µM has been demonstrated. Detection of PrPC in spiked blood plasma has been achieved and demonstrated a recovery of 85%.
 
5:00        カートリッジ型ポイントオブケアHIVウィルス量検査機器に対応するノースピン試料調製技術
Shan Gao, PhD, Senior Chemical Engineer, Wave 80 Biosciences, Inc.*
Porous polymer monoliths (PPMs) were developed to prepare nucleic acids from HIV virion-spiked whole blood or plasma samples for quantification of viral RNA using both standard qRT-PCR assays and a novel high-sensitivity bipartite signal amplification assay (BSAA). The efficiency of optimized in-cartridge, glycogen-mediated and monolith-based extraction of viral RNA was approximately 50%. This is comparable to standard centrifuge-based methods involving either phenol-chloroform extraction s or commercial spin columns. The new sample preparation method is particularly well suited for the development of point-of-care HIV viral load testing systems with sophisticated functionality and low cost.
*In collaboration with: F.Wang, H.Negussie, J.Frey, A.Arsham, A.Droitcour, L.Mazzola, D.Laser, Wave 80 Biosciences; and A.Fan, C.Klapperich, Boston University
 
5:30        出展者/スポンサーによるプレゼンテーション
 
6:00        1日目終了
 
 
 
2013年5月10日(金)
 
8:00        展示会/ポスター発表の見学、コーヒー
 
9:00        自己完結型体外診断カートリッジ
David Wright, CEO, Wi Medical Device Development, Inc.
This talk is aimed at the methods of designing a fully integrated self-contained IVD cartridge. The evolving world of complex IVD test cartridges is moving towards a self-contained format, where the fluidic assay is contained, from sample pre to waist containment. The need for this format is based on taking complex assays from the lab into the field, where persons of moderate skills may execute a test with confidence, in a short period of time, at acceptable cost. The world of diagnostics is changing the world of applied therapy, and thusly, the cost of healthcare.
 
9:30        臨床試料からライム病ボレリアの分子を直接検出するための改良された試料調製および標的濃縮技術
Mark W. Eshoo, PhD, Director of New Technology Development, Ibis Biosciences Inc., an Abbott Company
Abstract is not available at time of publishing. Please visit www.KnowledgeFoundation.com for the latest Program updates.
 
10:00    MEMS−マイクロ流体工学の統合基準と設計:磁気ベース試料濃縮/検出技術の例
Mark Tondra, PhD, President, Diagnostic Biosensors
Commercialization of Point of Care (POC) and integrated Lab on a Chip (LOC) products is advancing. One enabling factor is industry standards for microfluidic interfaces between two devices, like a detector chip and a concentrator chip. These standards facilitate the design of higher-level micro-scale systems using plug-and-play architectures. The state-of the art in MEMS-Microfluidics industry standards will be presented. Our own use of these standards in design of multi-tube fluidics interfaces in our biosensor products will be shown, as will applications of devices using magnetic microbeads for sample concentration and manipulation in microfluidic LOC systems.
 
10:30    休憩時間、展示会/ポスター発表の見学
 
11:00    LC-ESI MS/MS分析法による迅速な病原体同定と耐性予測のための完全に自動化された試料調製
Patrick Broyer, PhD, Senior Scientist, bioMérieux SA, France
This talk will present a new automated sample prep solution for extraction of proteins and peptides dedicated to LC-ESI-MS analysis. Protocol simplifications, new filtration device for urine and blood & automation of peptide digestion will be described to show the ability of this solution to move to a long 18h protocol to a short 1h fully automated protocol. Results will be showed demonstrating the capabilities to correctly identify resistant microorganisms from positive blood culture. The sample preparation automated prototype is already used in our laboratory providing within 1 hour peptides solution ready to be analyzed by LC-ESI-MS by batches of 30 samples per run.
 
11:30    複雑な環境マトリクスから生物学的粒子の分別と濃縮を行なう新たな膜ベースシステムの開発
Andy Page, President & CTO, InnovaPrep LLC
InnovaPrep LLC will present an update on development of a novel, integratable, membrane-based fractionation and concentration system, under a current Department of Homeland Security Phase II SBIR. Powerful, automated sample preparation techniques are needed for improved detection and identification of rare biological particles in complex environmental samples by autonomous biodetection systems. A novel cartridge containing a series of sharp-cut membrane filters in order of decreasing pore size, along with integrated pneumatic valves and fluid paths, are used to rapidly separate particles present in up to 20 mL of environmental sample by size into separate fractions containing large environmental particles, bacteria, viruses and DNA, and proteins. Each captured fraction is then eluted into separate concentrated volumes of less than 200 µL. Program goals, hurdles, and performance data will be presented.
 
12:00    次世代シーケンシングのための自動化された試料調製および分析技術
Numrin Thaitrong, PhD, Researcher, National Center for Genetic Engineering and Biotechnology / Sandia National Labs
Thorough characterization of next-generation sequencing library is a required process in the sample preparation workflow. However, this built-in quality control feature is currently not available for small-scale library construction platforms. We have developed an integrated droplet-based digital microfluidics (DMF) architecture with capillary interface to handle sub-microliter sample processing and analysis. The platform providing size distribution and quantization of the library can be coupled to the existing modular DMF “hub” to automate start-to-finish library construction.
 
12:30    昼食
 
2:00        臨床試料の収集、搬送、調整の改善
Marek Smieja, MD, PhD, Associate Professor, Dept of Pathology and Molecular Medicine, McMaster University, Canada*
The development of point of care diagnostic tests for common infectious diseases needs parallel improvements in the ease of collection of clinical samples, and appropriate transport media for in-tube sample stabilization and/or extraction. We studied the effectiveness and reproducibility of Copan’s FLOQSwabs™ for sample collection for respiratory, gastrointestinal, and genitourinary diseases. We determined the feasibility and performance characteristics of self-collected, less-invasive diagnostic samples; evaluated the robustness of transport media (dry swabs, UTM, eNAT, ESwab, MSwab, Cymol) under differing temperature and time conditions; and determined the performance characteristics of various media for antigen-based and molecular diagnostic tests.
*In collaboration with: Copan Diagnostics
 

2:30 Integrated PureLyse® Sample Preparation
Bruce Irvine, CTO, Claremont BioSolutions LLC
Claremont BioSolutions has created a novel method of rapid sample preparation that is entirely disposable. It provides mechanical cell lysis and simultaneously extracts nucleic acids or proteins. Nucleic acid can be extracted in three to five minutes with two to three steps. The disposable PureLyse® device is comprised of a micro-motor and vane that agitate particles at unusually high shear forces. As cells are lysed, nucleic acid binds to the lysing particles. This system delivers high yield RNA preparation as well. This system is also used to very effectively homogenize tissue. The miniature disposable nature of this flow-through cartridge lends itself to integration. Our flagship approach to integration is the OmniValveâ„¢ fluidic system, where we have embedded the PureLyse® chamber within a valve with up to six ports connecting the lysis and extraction cartridge to other chambers. This facilitates integration of DNA extraction with sample introduction, pre-filters, wash, elution, and amplification. This approach was very successfully applied to an NIH funded SBIR project developing a semi-integrated system for detecting Clostridium difficile, resulting in 100 % specificity and 96% sensitivity as compared to standard enzyme immunoassay of stool samples.

 
3:00        全血中での安定的な遺伝子発現:ヒト血液試料の常温処理による高品質RNAの精製
Vasco Liberal, PhD, Scientist, Biomatrica, Inc.
Expression profiles from blood samples are increasingly used to diagnose, monitor and treat diseases, requiring reliable RNA preservation during sample collection, transport and storage. Transcription profiles can change rapidly, potentially dictating inadequate treatment. Biomatrica has developed a blood collection device that stabilizes RNA in blood cells during ambient temperature sample handling and storage. With its coupled RNA purification kit, it provides a complete solution from blood collection to RNA purification, achieving high yields and great RNA quality, with unaltered gene expression for 14 days of room temperature storage, which can prove of extreme value for better patient treatment.
 
3:30        出展者とスポンサーのプレゼンテーション/優れたポスター発表の紹介
 
4:00        閉会の挨拶、学会終了

 


この学会では、企業や政府機関、大学などの研究者によるポスター発表が行なわれます。ポスター発表をご希望の方は、学会資料に掲載する1ページの要旨(8.5インチx 11インチ、余白1インチ)を2013年4月5日までに電子メールでお送りください。なお、ポスター発表の応募は4月20日まで受け付けますが、要旨を学会資料に掲載することはできません。

ポスターボードの大きさ:
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ご注意: ポスター発表に応募される場合には、学会への参加登録が必要であり、事前に登録料およびポスターボード予約料をお支払いいただいた時点でポスターボード掲示スペースの予約が完了します。
 
展示会への出展

この学会では、出展とスポンサーに関するさまざまなオプションが用意されています。特に好評を得ている価値の高いオプションとしては、下記のものがあります。利用可能なすべてのオプションが記載されたリストをご覧になりたい場合には、このウェブページの上部にある出展についてのPDFリンクをクリックするか、当社までお問合せください。

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パッケージの内容:
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A block of rooms has been allocated at the conference hotel. Please make your reservations by April 3, 2013 to obtain this rate. When making reservations, please refer to the Knowledge Foundation. Contact The Knowledge Foundation if you require assistance.

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