Cambridge Healthtech Institute’s 14th Annual

Difficult-to-Express Proteins
( 発現困難なタンパク質 )

Overcoming Expression Challenges



Proteins are each unique and bring unique challenges when attempting to tame them into submission. CHI’s 14th annual Difficult-to-Express Proteins conference examines the challenges researchers encounter when striving for high-yield production of “difficult-to-express” proteins (DTEPs) and the strategies and technologies that have proven successful in overcoming those challenges. Some of the difficulties encountered include solubility, proper folding, inability to crystallize and aggregation and formation of inclusion bodies. Researchers employ a range of problem-specific solutions to achieve expression including genetic modifications, manipulating how a target protein is produced and employing protein tags. In addition, the identification of DNA coding sequences along with the use of high-throughput approaches has brought about significant improvements. The Difficult-to-Express Proteins conference provides the latest developments in improving yield for DTEPs through Case Studies and breakthrough data.


Coverage will include, but is not limited to:


  • Taming difficult-to-express proteins
  • High-yield production of “difficult-to-express” proteins 
  • Membrane proteins (GPCRs, ion channels, etc.)
  • Caspases 
  • Proteases
  • Kinases
  • Genetic engineering
  • Gene Expression
  • Translation
  • Secretion
  • Re-Engineering
  • Catalytic activity
  • Physical properties
  • Amino acid sequence
  • Disulfide-rich proteins 
  • Disulfide bond stability
  • Overcoming mis-folding
  • Aggregation
  • Crystallization
  • Intended use
  • Scaling-up production
  • Cost of scale-up
  • Developing production-scale processes
  • Cell-free protein expression
  • Achieving soluble proteins
  • Tagged or fused proteins
  • To express in the cytoplasm, periplasm or extracellular in the medium?
  • Supporting tools and technologies
  • Ensuring quality


* 不測の事態により、事前の予告なしにプログラムが変更される場合があります。

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