liquidbiopsysummit 

The 2nd Annual Liquid Biopsy Summit
-第2回液体生検サミット-
開催日:2017年6月21-23日
開催地:米国カリフォルニア州サンフランシスコ、Hotel Kabuki
  • Final Agenda Now Available

生体分子医療の分野がかつてない盛り上がりを見せています。特に注目を集めているのは、1)体内の複数の組織から抽出された血中循環腫瘍細胞 (CTC) 、セルフリー核酸 (cfNA) 、細胞外小胞を含む生体液の最新の研究成果に基づく究明、2)生体分子の役割を分析可能な感度と精度の高い次世代シーケンシング (NGS) 技術の急速な発展と分子診断検査室での急速な普及、3)健康体、病体、治療反応での生体分子の役割を分析するための研究の進展、などの動きです。しかし、生体液ベースのDNA/RNA分析法にまつわる不安定さは払しょくされておらず、液体生検を幅広い用途で日常的に利用できるようにするためには、この問題の解決が不可欠です。今度のLiquid Biopsy Summitでは、この分野の専門家が一堂に会し、プロセスと技術の改良を進めて、分子液体生検を将来のプレシジョンメディシンの支柱にするための方策などについて議論します。


Wednesday, June 21

11:00 am Short Course Registration

11:30 am - 2:30 pm Pre-Conference Lunch Short Course*

SC1: Blood from Patient - Needle - Freezer - Analysis - Diagnosis: Biobanks and Their Critical Role in Moving Precision Medicine Forward

* Separate registration required.

2:00 Main Conference Registration

Precision Medicine: Sample & Standards

3:00 Organizer's Opening Remarks

Mary Ann Brown, Executive Director, Conferences, Cambridge Healthtech Institute

3:05 Chairperson's Opening Remarks

3:15 FEATURED PRESENTATION: Pre-Analytical Variables in Blood Collection Impact on Precision Medicine

Carolyn_ComptonCarolyn Compton, M.D., Ph.D., Professor of Life Sciences, School of Life Sciences, Arizona State University; Professor of Laboratory Medicine and Pathology, Department of Pathology, Mayo Clinic School of Medicine

This presentation focuses on the importance of evidence-based standardization of the pre-analytical steps in biospecimen acquisition and handling for liquid biopsies in order to assure reproducible, clinically meaningful analysis results. The work of the Pre-analytics for Precision Medicine Project Team of the College of American Pathologists (CAP), the American Society of Clinical Oncology (ASCO)-CAP Liquid Biopsies Working Group and the Beau Biden Cancer Moonshot Blood Profiling Atlas in Cancer (Blood PAC) will be highlighted.

4:00 CO-PRESENTATION: Improving the Quality of Circulating Tumor DNA Measurements by a Multi-Laboratory Assessment of Expert-Designed Reference Materials

Kenneth_ColeKenneth D. Cole, Ph.D., Group Leader, Bioassay Methods, National Institute of Standards and Technology (NIST)


Russell_GarlickRussell K. Garlick, Ph.D., CSO, SeraCare Life Sciences, Inc.

Analytical validation of ctDNA assays must be accomplished before clinical validation. ctDNA assays present unique sensitivity and specificity challenges due to the biochemical nature of circulating cell-free DNA and multiple analytical measurement techniques. This presentation discusses the design, development and characterization of expert-designed reference materials for use in a multi-lab assessment of ctDNA measurements. Commutability to patient samples and concordance between analytical methods will be discussed. 

4:45 PANEL DISCUSSION: Enhancing the Science and Clinical Utility of Liquid Biopsies

All agree that the potential of liquid biopsies will allow for detection of disease faster, diagnosis of disease earlier, and tracking of disease progression and treatment response more efficiently.

This panel discusses progress in:

  • collection, preservation, and storage of biosamples
  • advances in detection technologies
  • determining reference materials and standards
  • developing safe and effective blood profiling diagnostics
  • creating an open database of liquid biopsy results

Panelists:

Kenneth D. Cole, Ph.D., NIST

Carolyn Compton, M.D., Ph.D., Arizona State University & Mayo Clinic School of Medicine

Russell K. Garlick, Ph.D., SeraCare Life Sciences, Inc.

Additional Panelists to be Announced

5:30 Welcome Reception in the Exhibit Hall with Poster Viewing

6:30 Close of Day

Thursday, June 22

8:00 am Morning Coffee

Precision Oncology: Cells & Cell Targets

8:45 Chairperson's Remarks

Rebecca (Becky) Suttmann, MS, Senior Scientific Researcher, Oncology Biomarker Development, Genentech, Inc.

8:50 High-Content Analysis of Prostate Cancer Cells in Bone Marrow and Blood

James_HicksJames Hicks, Ph.D., Professor, Department of Biological Sciences, University of Southern California

It is rapidly becoming an article of faith that the 'Liquid Biopsy,' using a suite of cellular and molecular assays of blood or other body fluids, will become a key component in our progress toward precision medicine and personalized treatment of cancer and other diseases. An array of blood-based assays is being developed that can provide a real-time window into the course of the disease and its response to treatment. HD-SCA is an integrated proteogenomic liquid biopsy platform for assessing cancer heterogeneity at both the cellular and molecular levels. We will describe its integration into the Blood Profiling Atlas of Cancer (BloodPAC), a joint industry and academic effort spawned from the Biden Cancer Moonshot initiative of 2016.

9:20 Retrospective Analysis of CellSearch CTC Enumeration Data across Clinical Trials

Becky_SuttmanRebecca (Becky) Suttmann, MS, Senior Scientific Researcher, Oncology Biomarker Development, Genentech, Inc.


9:50 Multi-Component Liquid Biopsy Enabled by High-Throughput Optical Scanning Technologies

Zheng_AoZheng Ao, Ph.D., Postdoctoral Fellow, Biosciences Division, SRI International

Liquid biopsies provide a non-invasive means to access molecular and genetic information of the tumor. Using the high-throughput optical scanning technology here at SRI, we aim to interrogate not only the tumor cells in circulation (CTCs) but also components of the tumor microenvironment such as cancer-associated fibroblasts (CAF), tumor-associated lymphocytes (TAL) and extracellular vesicles (EVs). This multi-component analysis is more representative of the whole tumor than CTCs alone and will better enable us to put the puzzle together to understand the tumor through a tube of blood.

10:20 Coffee Break in the Exhibit Hall with Poster Viewing

Diagnosing & Tackling Other Circulating Targets

11:00 Liquid Biopsy in Neurovascular Inflammation

Winny_YunJ. Winny Yun, Graduate Assistant, JS Alexander Lab, Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center

Inflammatory cytokines promote cerebrovascular stress in several neurodegenerative diseases, including multiple sclerosis (MS), and may provoke endothelial disturbances, contributing to blood-brain barrier breakdown and exacerbation of disease. We recently identified lymphatic endothelial-specific proteins in human brain tissue and in the serum, with altered expression in MS patient samples. This indicated the possibility that circulating lymphatic biomarkers may be derived from brain endothelial cells in the form of circulating microparticles.

11:30 Detection of Human Cardiomyocyte Death Using Cell-Free DNA

Hai Zemmour, Research Scientist, Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School

I present a totally new approach for studying onset cell type-specific death, especially cardiomyocyte degeneration in various pathological contexts, and envision a new type of blood test that aimed sensitive detection of tissue damage without a priori suspicion of disease. I believe that such a tool will have broad utility in diagnostic medicine and in the study of human biology.

12:00 pm Sponsored Presentation (Opportunity Available)

12:30 Session Break

12:45 Luncheon Presentation (Sponsorship Opportunity Available) or Enjoy Lunch on Your Own

Precision Oncology: Monitoring Therapy Response

2:00 Chairperson's Remarks

2:05 Liquid Biopsies in Cancer Immunotherapy Clinical Drug Development

Shidong_JiaShidong Jia, M.D., Ph.D., Founder & CEO, Predicine, Inc.

Cancer immunotherapy offers great promise where biomarkers have been shown to predict therapy outcome in various types of cancer patients. The talk describes the development and clinical application of a next-generation sequencing-based liquid biopsy test to support cancer immunotherapy clinical trials.

2:35 Microfluidic Cell Tethering to Rapidly Measure Drug Responses in Patient Tumor Cells

Stuart_MartinStuart S. Martin, Ph.D., Professor of Physiology, Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine

Non-adherent tumor cells display four phenotypes that are indicative of metastatic potential (microtentacles, clustering, deformability, stem cell sphere formation). However, technical challenges prevent high-resolution imaging of non-adherent tumor cells. Integrating hydrophobic lipids into an engineered microfluidic surface tethers tumor cells while preserving the dynamic behaviors of non-adherent tumor cells. This technology enables rapid testing of patient cells for responses to drug treatments that could influence CTC reattachment during metastasis. 

3:05 Sponsored Presentation (Opportunity Available)

3:20 Refreshment Break in the Exhibit Hall with Poster Viewing

Precision Oncology: Diagnosis & Prognosis

4:00 FEATURED PRESENTATION: Epigenetic and Genetic Analysis of Circulating Biomarkers for Prostate Cancer Prognosis

Tim_HuangTim H-M Huang, Ph.D., Professor & Chair, Department of Molecular Medicine, University of Texas Health Science Center at San Antonio

A continuous rise of serum PSA in patients after receiving radical prostatectomy is indicative of recurrent prostate cancer, or biochemical recurrence (BCR). Androgen-deprivation therapies appear effective for these patients when the BCR can be detected earlier. The emergence of circulating biomarkers provides a new opportunity to non-invasively monitor disease recurrence and patients' treatment responses. Shed into the bloodstream of cancer patients, these biomarkers can be derived from cell-free DNAs (cfDNAs) and circulating tumor cells (CTCs). We have recently found that increased cfDNA methylation of androgen-biosynthesis loci is frequently associated with rising PSA in prostate cancer patients. In a separate study, we have observed that increased cell rigidity, adhesiveness and deformability of CTCs represent novel biomarkers for BCR. These altered nanomechanical properties are often accompanied with the acquisition of an epithelial-mesenchymal transition (EMT) phenotype in these circulating cells of advanced prostate cancer patients. Together, this integrative assay of exfoliated cell sources can improve detection of recurrent prostate cancer and is useful for monitor disease progression.

4:30 Proteomic Alterations of Tumor Cell Adhesion Are Detectible in Patient Biofluids and Report on Their Outcome

Andries_ZijlstraAndries Zijlstra, Ph.D., Associate Professor, Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center

Proteins mechanistically involved in tumor cell migration and metastatic dissemination have the potential to serve as markers of invasive disease and predict patient outcome. The activity of the cell adhesion molecule ALCAM (Activated Leukocyte Cell Adhesion Molecule) is regulated dynamically through proteolytic release of its extracellular domain. As such, the detection of shed ALCAM acts as a prognostic biomarker for solid tumors.

5:00 Heterogeneity of Cancer-Derived Extracellular Vesicles: The Emerging Role of Large Oncosomes

Dolores_Di_VizioDolores Di Vizio, M.D., Ph.D., Associate Professor, Pathology & Laboratory Medicine, Biomedical Sciences, Cancer Biology Program, Cedars-Sinai Medical Center; Associate Professor, Medicine, University California, Los Angeles; Assistant Professor, Boston Children's Hospital, Harvard Medical School

Extracellular vesicles (EVs) are important mediators of intercellular mechanisms as they can shuttle from one cell to another a reservoir of functional molecules. EVs are being recognized as appealing biomarkers of diagnosis and prognosis. We have demonstrated that the abundance of large oncosomes in the circulation and in tissues correlates with advanced disease in mouse models and human subjects, and that these vesicles are promising candidates for liquid biopsy approaches through large-scale profiling of protein, DNA and mRNA.

5:30 Short Course Registration

5:45 - 8:45 Dinner Short Course*

SC2: Innovative Imaging Technologies for Patient CTCs

* Separate registration required.

Friday, June 23

7:30 am Breakfast Breakout Discussion Groups

Chew over breakfast and provocative discussion topics with your peers. These are moderated discussions with brainstorming and interactive problem-solving, allowing conference participants from diverse backgrounds to exchange ideas and experiences and develop future collaborations around a focused topic.

Translation into the Clinic

9:00 Chairperson's Remarks

Gary Spitzer, M.D., Director, Clinical Validity and Clinical Utility Evaluation, MolDX, Palmetto GBA

9:05 Clinical Utility Needs of ctDNA Assays versus Research Utility

Gary Spitzer, M.D., Director, Clinical Validity and Clinical Utility Evaluation, MolDX, Palmetto GBA

I present design of prospective transparent registries to validate the clinical utility of a test in a clear intent to study patient population. I also discuss obstacles of existing registry and trial design and present ideas on how costs can be diminished through non-traditional data extraction techniques, but with emphasis on transparency, scientific independence and open data collection.

9:35 Liquid Biopsies in Oncology and the Current Regulatory Landscape

Mark W. Linder, Ph.D., DABCC, Professor, Department of Pathology and Laboratory Medicine, University of Louisville School of Medicine

There is a need to detect cancer earlier, guide therapies and monitor progress during treatment. However, solid tissue biopsy is at times limited by the invasiveness and tumor size. A liquid biopsy can overcome these barriers and allow samples to be collected over time. Liquid biopsies include the analysis of circulating tumor cells (CTCs) and/or cell-free nucleic acids obtained from a variety of biofluids.

10:05 Validating Liquid Biopsies in Cancer: How Sensitive Is Clinically Meaningful?

John Beeler, Ph.D., Vice President, Corporate and Business Development, Inivata, Inc.

10:20 Coffee Break in the Exhibit Hall with Poster Viewing

10:45 Colvera™: A ctDNA Liquid Biopsy Assay for Colorectal Cancer - Our Journey from Discovery to the Clinic

Jason_RossJason Ross, Ph.D., Senior Research Scientist, Health and Biosecurity, CSIRO

We have developed Colvera™, a ctDNA liquid biopsy assay to diagnose and monitor colorectal cancer. The assay is based upon detecting circulating tumor DNA from two genes methylated in colorectal cancer, but unmethylated in non-neoplastic tissue or blood. I discuss the journey we and our collaborators undertook: from biomarker discovery and validation, to assay design and then to clinical trials and the launch of a commercial lab test.

11:15 Development and Validation of a Pan-Cancer Liquid Biopsy Test: Criteria and Considerations

Honey_ReddiHoney V. Reddi, Ph.D., FACMG, Clinical Laboratory Director, The Jackson Laboratory for Genomic Medicine

The presentation delineates the criteria that went in content evaluation, steps involved in validation including sample extraction methods, and also covers the limitations and challenges involved in the process.


11:45 Sponsored Presentation (Opportunity Available)

12:15 pm Session Break

12:30 Luncheon Presentation (Sponsorship Opportunity Available) or Enjoy Lunch on Your Own

New Tools: Advancing Detection for Liquid Biopsies

1:30 Chairperson's Remarks

Lynne Bemis, Ph.D., Chair, Biomedical Sciences, University of Minnesota


1:35 KEYNOTE PRESENTATION: New Tools for Liquid Biopsies: Microfluidic Platforms for the Isolation of CTCs, cfDNA and Exosomes

Steven_SoperSteven A. Soper, Ph.D., Foundation Distinguished Professor, Department of Chemistry, Department of Mechanical Engineering and Bioengineering; Director, Center of BioModular Multi-Scale Systems for Precision Medicine, The University of Kansas

Liquid biopsies are generating great interest within the medical community due to the simplicity for securing important biomarkers to manage complex diseases, such as cancer-related diseases. These circulating markers consist of CTCs, cfDNA and exosomes. We are developing a suite of microfluidic devices that can process whole blood directly and are engineered to efficiently search for a variety of disease-associated liquid biopsy markers from divergent subpopulations comprising the tumor microenvironment.

2:20 Optofluidic Systems for On-Chip Detection of Cancer and Infectious Disease Biomarkers

Holger_SchmidtHolger Schmidt, Ph.D., Professor, Electrical Engineering; Narinder Kapany Chair of Optoelectronics; Director, W.M. Keck Center for Nanoscale Optofluidics, University of California, Santa Cruz

Optofluidic devices combine microfluidics with integrated optics, creating a single chip-based system for both preparation and analysis of biological samples such as liquid biopsies. I describe a versatile, optofluidic platform using liquid-core optical waveguides for optical analysis of single biomolecules. Examples for its capabilities include amplification-free detection of Ebola RNA, multiplex detection of single influenza viruses and cancer DNA biomarkers starting with a whole blood sample.

2:50 Sizing Up Cancer in Cell-Free DNA

Hunter_UnderhillHunter R. Underhill, M.D., Ph.D., Assistant Professor, Pediatrics, Division of Medical Genetics, University of Utah

Detection of cell-free DNA derived from non-metastatic solid tumors remains challenging. Identification of tumor-associated variants in cell-free DNA that occur at very low frequency (<1%) requires methods with ultra-high sensitivity. Here, the role of size selecting for specific cell-free DNA fragment lengths to improve detection of solid tumors will be discussed. Size selection will be considered within the context of mutant allele enrichment and potential effects on next-generation sequencing.

3:20 Networking Refreshment Break

3:30 Making and Using RNA Standards: Can You Trust Your Genome-Scale Measurements?

Sarah_MunroSarah A. Munro, Ph.D., Team Leader, Genome-Scale Measurements Group, Joint Initiative for Metrology in Biology, National Institute of Standards and Technology (NIST)

When you're assaying thousands of molecules at the same time, how do you know that you have the right answers? To make the 21st century of biology a reality, we need genome-scale measurement results we can trust. In our Joint Initiative for Metrology in Biology (JIMB), we are working to systematically address these needs with RNA spike-in controls, mixture samples, and turn-key analysis tools.

4:00 tRNA Fragments in Accessible Samples: Expression, Biogenesis and Function

Lynne_BemisLynne Bemis, Ph.D., Chair, Biomedical Sciences, University of Minnesota

tRNA fragments are common in RNA-seq libraries and were originally thought to be breakdown products. We identified a tRNA fragment that is present at high levels in the urine of patients with ovarian cancer and we have now shown that its cleavage is regulated by the RNase, angiogenin. The function of this and other tRNA fragments in cancer cell lines will be described.

4:30 Multitarget Serum MicroRNA Assays for Early Detection of Lung and Gastric Cancers

Lihan_ZhouLihan Zhou, Ph.D., CTO & Founder, MiRXES Pte Ltd.

Lung and gastric cancers rank 1st and 3rd in mortality globally. Current screening methods of LDCT and gastroscopy are limited by specificity, invasiveness and cost. Using a proprietary ultra-sensitive qPCR technology, we have developed serum miRNA assays to serve as an alternative for blood-based lung and gastric cancer screening. These assays have been validated in multi-center studies with encouraging accuracy in detecting Stage I and II cancers (AUC>0.9).

5:00 Close of Conference


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